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Fig. 6 | BMC Microbiology

Fig. 6

From: Development and validation of a CRISPR interference system for gene regulation in Campylobacter jejuni

Fig. 6

Assessing the impact of CRISPRi-based repression of flaA and flaB independently in M1Cam and 81–176 motility. Two sgRNA target locations unique to each of flaA and flaB were identified (A). Motility analysis of CRISPRi transformants targeting flaA (B) showed a significant motility reduction in M1Cam flaA_761 and flaA_1662, but no reduction in 81–176 flaA_761 and flaA_1662. Motility plating of CRISPRi transformants targeting flaB (C) showed a small but significant reduction in motility in 81–176 flaB_791 and flaB_1592, and a significant decrease in motility in M1Cam flaB_1592 but no significant decrease in M1Cam flaB_1662 (* = p < 0.05, ** = p < 0.01, One-way T-test, N = 3 biological replicates, error bars = SD)

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BMC Microbiology

ISSN: 1471-2180

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