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Fig. 5 | BMC Microbiology

Fig. 5

From: Development and validation of a CRISPR interference system for gene regulation in Campylobacter jejuni

Fig. 5

Assessing the impact of CRISPRi-based repression of flgR on M1Cam and 81–176 motility. Five sgRNA target locations (A) were identified in flgR in M1Cam and 81–176. Motility plating of CRISPRi transformants (B) showed a significant decrease in motility (* = p < 0.05, ** = p < 0.01 and *** = p < 0.001, One-way T-test, N = 3 biological replicates, error bars = SD) for all sgRNA locations except M1Cam flgR_559 and 81–176 flgR_647, which showed no significant difference in motility

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