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Fig. 3 | BMC Microbiology

Fig. 3

From: Development and validation of a CRISPR interference system for gene regulation in Campylobacter jejuni

Fig. 3

Quantification and comparison of AstA activity in 81–176, M1Cam and M1Cam∆CRISPR CRISPRi transformants using a nitrophenol assay. A significant decrease in AstA activity was demonstrated for each sgRNA in all CRISPRi transformants (A, B and C) in comparison to each respective WT (**** = P < 0.0001, ANOVA, N = 3, error bars = SD). Mean percentage decrease calculations for each CRISPRi transformant with respect to the WT in each strain showed no significant difference in CRISPRi efficacy between M1Cam and M1Cam∆CRISPR (D). All sgRNA target locations showed > 78% decrease in detectable AstA activity across all strains. No significant difference in sgRNA efficacy was detected between strains except for astA_85 and astA_1091 between M1Cam and 81–176 (* = p < 0.05, 2-way ANOVA, error bars = SD)

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BMC Microbiology

ISSN: 1471-2180

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