Fig. 1

Activity screening of candidate C. jejuni promoters in M1Cam and 81–176 using a LacZ reporter system. Four C. jejuni promoters, pPorA (A,i), pCatA (A,ii), pCas9 (A,iii) and pMetK (A,iv) were each inserted into a lacZ Kan^r reporter construct (v). The reporter constructs were transformed into M1Cam and 81–176 and screened using a β-galactosidase assay (B and C, respectively). In both strains, pPorA demonstrated the greatest activity, followed by pCat and pCas9 which displayed similar activities, and pMetK, which had the lowest activity. **** = p < 0.0001, *** = p < 0.001. N = 3 biological replicates, Statistics performed using an unpaired t-test, error bars = SD