Fig. 6
From: Carboxypeptidase G and pterin deaminase metabolic pathways degrade folic acid in Variovorax sp. F1
Enzymatic properties of rPDA from Variovorax sp. F1. A SDS-PAGE gel. Lanes: M, molecular weight marker; rPDA, Recombinant PDA (2 μg) from Variovorax sp. F1 strain. B HPLC analyses of PDA reactions containing 0.01 μg mL− 1 rPDA and 1 mM substrate (left, FA; right, PA) at 30 °C for 2 min. Traces: 1, substrates; 2, products; 3, reaction. C Initial velocity of rPDA reaction determined to calculate Km and kcat values of rPDA for FA (left) and PA (right) as substrates. Data were fitted to Michaelis-Menten equation