Fig. 2
Construction of plsC4inact. and plsC5inact. mutants. A The wild-type (upper) and mutated (lower) nucleotide and amino acid sequences of catalytic site-coding regions. The small characters indicate the mutated nucleotides. A SalI site in the wild-type plsC5, of which loss indicates the successful mutation, is shaded. Raw sequencing data for the mutated sequences are shown at the bottom. B The growth curves of parent (black), plsC4inact. (blue), and plsC5inact. cells (red). Solid and broken lines indicate growth profiles at 18 °C and 4 °C, respectively