Table 9 Steepest ascent path experimental design and results
Test | medium volume (ml) | initial pH | fermentation temperature (°C) | Titer (mg/l) |
|---|---|---|---|---|
1 | 60 | 6.0 | 30.0 | 6363 |
2 | 55 | 6.5 | 31.0 | 6889 |
3 | 50 | 7.0 | 32.0 | 7467 |
4 | 45 | 7.5 | 33.0 | 7148 |
5 | 40 | 8.0 | 34.0 | 6422 |
6 | 35 | 8.5 | 35.0 | 5012 |
7 | 30 | 9.0 | 36.0 | 4566 |