Table 7 Design and results of PB experiments
Runs | A | B | C | D | E | F | Titer (mg/l) | |
|---|---|---|---|---|---|---|---|---|
Experimental | Predicted | |||||||
1 | 40(-1) | 7.0(+ 1) | 1.5(-1) | 6(-1) | 120(-1) | 35.0(+ 1) | 4200 | 4237 |
2 | 60(+ 1) | 5.0(-1) | 1.5(-1) | 6(-1) | 180(+ 1) | 35.0(+ 1) | 3048 | 2986 |
3 | 40(-1) | 5.0(-1) | 1.5(-1) | 6(-1) | 120(-1) | 25.0(-1) | 3133 | 3061 |
4 | 60 (+ 1) | 5.0(-1) | 2.5(+ 1) | 6(-1) | 120(-1) | 25.0(-1) | 2333 | 2380 |
5 | 60 (+ 1) | 5.0(-1) | 2.5(+ 1) | 8(+ 1) | 120(-1) | 35.0(+ 1) | 2667 | 2699 |
6 | 40 (-1) | 5.0(-1) | 2.5(+ 1) | 8(+ 1) | 180(+ 1) | 25.0(-1) | 3267 | 3188 |
7 | 60 (+ 1) | 7.0(+ 1) | 1.5(-1) | 8(+ 1) | 120(-1) | 25.0(-1) | 2600 | 2512 |
8 | 40 (-1) | 7.0(+ 1) | 2.5(+ 1) | 8(+ 1) | 120(-1) | 35.0(+ 1) | 3867 | 3926 |
9 | 60 (+ 1) | 7.0(+ 1) | 2.5(+ 1) | 6(-1) | 180(+ 1) | 35.0(+ 1) | 3200 | 3278 |
10 | 60 (+ 1) | 7.0(+ 1) | 1.5(-1) | 8(+ 1) | 180(+ 1) | 25.0(-1) | 3000 | 2943 |
11 | 40 (-1) | 5.0(-1) | 1.5(-1) | 8(+ 1) | 180(+ 1) | 35.0(+ 1) | 3667 | 3602 |
12 | 40 (-1) | 7.0(+ 1) | 2.5(+ 1) | 6(-1) | 180(+ 1) | 25.0(-1) | 3333 | 3302 |