Table 10 Experimental design and response values from CCD
Run | Factors | Titer (mg/l) | |||
|---|---|---|---|---|---|
A:medium volume (ml) | B:initial pH | F:fermentation temperature (°C) | Experimantal | Predicted | |
1 | + 1.682(58) | 0(7.0) | 0(32) | 6199 | 6235 |
2 | 0(50) | 0(7.0) | 0(32) | 7470 | 7398 |
3 | + 1(55) | -1(6.5) | + 1(33) | 5990 | 6026 |
4 | 0(50) | 0(7.0) | 0(32) | 7691 | 7639 |
5 | + 1(55) | -1(6.5) | -1(31) | 5782 | 5814 |
6 | -1(45) | -1(6.5) | -1(31) | 5733 | 5688 |
7 | -1.682(42) | 0(7.0) | 0(32) | 5720 | 5673 |
8 | 0(50) | -1.682(6.2) | 0(32) | 6132 | 6185 |
9 | 0(50) | 0(7.0) | -1.682(30.3) | 5619 | 5725 |
10 | + 1(55) | + 1(7.5) | -1(31) | 6353 | 6279 |
11 | -1(45) | + 1(7.5) | -1(31) | 5561 | 5607 |
12 | + 1(55) | + 1(7.5) | + 1(33) | 6899 | 6823 |
13 | -1(45) | + 1(7.5) | + 1(33) | 5886 | 5936 |
14 | 0(50) | + 1.682(7.8) | 0(32) | 6341 | 6281 |
15 | 0(50) | 0(7.0) | + 1.682(33.7) | 6058 | 6090 |
16 | 0(50) | 0(7.0) | 0(32) | 7900 | 7983 |
17 | 0(50) | 0(7.0) | 0(32) | 7863 | 7793 |
18 | -1(45) | -1(6.5) | + 1(33) | 5659 | 5588 |
19 | 0(50) | 0(7.0) | 0(32) | 7893 | 7953 |
20 | 0(50) | 0(7.0) | 0(32) | 7937 | 8025 |