From: Isolation and identification of a new biocontrol bacteria against Salvia miltiorrhiza root rot and optimization of culture conditions for antifungal substance production using response surface methodology
Factors
Level
-1
+ 1
A: medium volume (ml)
50
70
B: initial pH
5.0
7.0
C: inoculum size (%)
1.5
2.5
D: fermentation time (d)
6.0
8.0
E: rotary speed (rpm)
120
180
F: fermentation temperature (°C)
25
35