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Fig. 5 | BMC Microbiology

Fig. 5

From: A microfluidic-based analysis of 3D macrophage migration after stimulation by Mycobacterium, Salmonella and Escherichia

Fig. 5

Migration of macrophages in 2.5 mg/ml collagen gels towards a gradient generated at the top (+ y axis, indicated by grey dots) with M. smegmatis bacterial fractions: non-stimulated control (gray), secreted protein (pink), inactivated by PFA (yellow), cell lysate (blue) and inactivated by heat (green). A Directionality of migration, where the radius is the number of cells migrating in each direction and normalized by the total number of cells per condition. B Percentage of macrophages as a function of the verticality of their migration and weighted by the total distance traveled. Those macrophages whose final position is above their initial position contribute to the bar graph exceeding the red line set at 0.5. The dots in each bar graph correspond to the percentage of macrophages whose final position is above or below their starting position, but not weighted by the total distance migrated. n (devices) = 9 (control), 5 (secreted protein), 5 (inactivated by PFA), 5 (cell lysate) and 5 (inactivated by heat)

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