Table 1 B. cereus strains, fungus and the plasmids used in this study
Name | Property |
|---|---|
B. cereus 0–9 | Endophytic bacteria isolated from wheat roots by us |
ΔamyC | The amyC gene mutant strain of B. cereus 0–9 |
ΔamyP | The amyP gene mutant strain of B. cereus 0–9 |
ΔamyS | The amyS gene mutant strain of B. cereus 0–9 |
ΔamySec | Mutant strain of B. cereus 0–9 with deletion of only the signal peptide coding region of the amylase gene |
ΔamyS/amyS | The complementary strain of ΔamyS |
B. cereus 0–9 (RFP) | The red fluorescent protein label strain of B. cereus 0–9 |
ΔamyS (RFP) | The red fluorescent protein label strain of ΔamyS |
ΔamyS/amyS (RFP) | The red fluorescent protein label strain of ΔamyS/amyS |
ΔamySec (RFP) | The red fluorescent protein label strain of ΔamySec |
E.coli BL21/pET28a-amyS | Strain expressing amyS without a signal peptide coding region |
R. cerealis | Fungus causing wheat sharp-eyespot |
Plasmid pMAD | Plasmid containing lacZ, Amp and Erm tags and used for the gene mutation process |
Plasmid pMADchi | Plasmid containing upstream and downstream fragments of chi gene in the polyclonal cleavage site of plasmid pMAD [30] |
Plasmid pAD123-Pgal | Plasmid used for the construction of complementation strains, containing Amp and Cm tag [31] |
Plasmid pET-28a | Plasmid used for gene expression, containing a km tag |