Fig. 1

Analysis of the amyS gene (FRY47_17030) signal peptide and smart domain. A Analysis of the AmyS protein signal peptide through SignalP 4.1. S-score: Each amino acid corresponds to 1 S value, with a high S value in the signal peptide region. C-score: Each amino acid will have a C value, with the highest C value at the shear site. Y-score: The Y value is a parameter considering the S and C values and is more accurate than the C value alone because there may be more than one higher C value in a series but only one shear site; the shear site has a steep S value and a high C value. B Analysis of the AmyS protein smart domain using SMART software. Positions 33–421 represent the α-amylase domain, which catalyzes the hydrolysis of (1–4)-α-D-glucosidic linkages in polysaccharides to remove successive α-maltose residues from the nonreducing ends of the chains in the conversion of starch to maltose