Fig. 6
From: Comparison of antibacterial and antibiofilm activity of bioactive glass compounds S53P4 and 45S5
Fluorescence microscopy and 3D reconstruction images of ex vivo biofilm formations of clinical samples. Biofilms were stained with Syto9 (green) and PI (red), and fluorescence microscopy images were captured at 40 × magnification. Scale bar: 50 µm. A-C 24 h baseline biofilm; E–G 48 h non treated control; I-K ex vivo biofilm treated with 200 mg/ml 45S5/32–125 µm; M–O ex vivo biofilm treated with 400 mg/ml 45S5/32–125 µm. D, H, L and P 3D reconstruction images were generated based on corresponding merged images by using ImageJ