Fig. 8

Caspase activity and protease inhibition during light organ apoptosis in gravity and LSMMG conditions at 10 h post-inoculation. The activity of a executioner caspases 3/7, b extrinsic initiator caspase-8, and c intrinsic initiator caspase-9 was measured in the light organ of symbiotic (sym) hatchlings at 10 h in gravity (blue) and modeled microgravity (red). d Apoptosis was quantified in symbiotic hatchlings at 10 h in both conditions following protease inhibitor treatment including pan-caspase inhibitor z-VAD-FMK (ZVAD), the caspase 8 inhibitor Ac-IETD-CHO (C8i), caspase 9 inhibitor Ac-LEHD-CMK (C9i), Pefabloc (Pefa) and dimethyl sulfoxide (DMSO) controls. Error bars are the standard error of the mean. Asterisks denote significant differences between datasets (* = p ≤ 0.05, ** = p ≤ 0.01). Comparisons that were not significant are labeled “ns”