Table 2 the specific primers of quantitative real-time PCR
From: Effects of artificial honey and epigallocatechin-3-gallate on streptococcus pyogenes
Detected virulence factor | Primer sequence(5’ → 3’) | Reference |
|---|---|---|
speB | F: TGACGCTAACGGTAAAGAAAACA R: GCCGCCACCAGTACCAAGAGC | Burton et al. (2006) [65] |
hasA | F: GTGTGTTAGCACAGACCTATCC R: CTTCGAAAATAGTCCATAAGGC | Petros et al. (2009) [66] |
luxS | F: ATGATAAAGTGTA R: TTAGATTACTGAG | Raja et al. (2014) [67] |
16S rRNA | F: AAGAGTTTGATCCTGGCTCAG R: GGTTACCTTGTTACGACTT | Hassan et al. (2015) [68] |