Fig. 3

Symptoms of fish tested for recursive infection. A Body chart of experimental fish in control group. B Abdominal cavity of fish in control group (white arrow). C Phosphorus loss in a large area of the skin (white arrow). D Hemorrhagic necrosis of the liver (aa white arrow); a large amount of fluid in the abdominal cavity (ab white arrow); and intestinal inflammation (ac white arrow). E Agarose gel electrophoresis of PCR products of vvhA and rtxA from the suspected V. vulnificus isolates. M, Marker (unit, bp); 1–4, PCR products of vvhA from V. vulnificus in samples from four diseased fishes in the 1 × 10⁷–1 × 10⁴ CFU mL⁻¹ groups; 5–8, PCR products of rtxA from V. vulnificus in samples from 4 diseased fishes in the 1 × 10⁷–1 × 10⁴ CFU mL.⁻¹ groups; 9, negative control; 10–11, positive control (PCR products of vvhA and rtxA from EPL 0201 isolate). Original agarose gel electropherogram (Fig. S6)