Fig. 2

Molecular identification and virulence gene detection of strain EPL 0201. A Phylogenetic tree of Vibrio spp. based on the 16S rRNA gene sequence constructed using the neighbor-joining method. Bootstrap values were based on 1000 replicates, and percentage bootstrap values are shown at each node. B Phylogenetic tree of Vibrio spp. based on gyrB sequence constructed using the neighbor-joining method. Bootstrap values were based on 1000 replicates, and percentage bootstrap values are shown at each node. C Agarose gel electrophoresis did not show the presence of vcgC and OmpU but showed the presence of vvhA, rtxA, and Wza in the PCR products. M, Marker; Neg, negative control. Original agarose gel electropherogram (Supplementary Fig. S6)