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Table 1 Bacterial strains, plasmids, and primers used in this study

From: A novel, non-GMO surface display in Limosilactobacillus fermentum mediated by cell surface hydrolase without anchor motif

 

Features or sequences

Source

Strains

 Escherichia coli DH5α

Cloning host; F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF) U169, hsdR17(rK- mK +), λ–

Biofact

 E. coli BL21 (DE3)

Expression host; F- ompT hsdSB(rB-mB-) gal dcm (DE3)

Real BioTech

 Lactiplantibacillus plantarum SK151

Display host, wild type

Our laboratory

 Lp. plantarum SK156

Display host, wild type

Our laboratory

 Limosilactobacillus fermentum SK152

Display host, wild type

Our laboratory

 Lm. mucosae LM1

Display host, wild type

Our laboratory

 Lactobacillus johnsonii PF01

Display host, wild type

Our laboratory

Plasmids

 pET21b ( +)

Expression vector; N-terminal 6His-tag, Ampr

 

 pSfGFP

pET21b ( +) carrying 6 His-tagged sfGFP gene, Ampr

This study

 pCSHA-sfGFP

pET21b ( +) carrying 6 His-tagged cshA-sfGFP fusion gene, Ampr

This study

 pCB4270B-sfGFP

Plasmid containing sfGFP gene

[45]

Primers

 C1

5′-CCCCATATGAAAAAAACACGCGCC-3′

This study

 C2

5′-ACCCTTTGACATGCGTTTATCAGGAACATAGTG-3′

This study

 CS1

5′-GTTCCTGATAAACGCATGTCAAAGGGTGAAGAA-3′

This study

 CS2

5′-GGGCTCGAGCTTGTATAATTCATCCATACC-3′

This study

 S1

5′-GGGGCTAGCATGTCAAAGGGTGAAGAA-3′

This study

 S2

5′-GGGCTCGAGCTTGTATAATTCATCCATACCATG-3′

This study

  1. Nucleotide sequences in bold are overlapping sequences for fusion PCR
  2. Enzyme restriction sites are underlined accordingly: NheI and XhoI