Fig. 6

Effect of metals and NaCl on Bgl3HB enzyme activity with p-nitrophenyl-β-D-glucopyranoside (pNPG) as substrate. The values represent the mean ± SD (n = 3). a The reactions were performed by incubating purified enzyme with 1 mM and 5 mM of various metal ions (Cu²⁺, Ca²⁺, Ni²⁺, Mg²⁺, K⁺, Al³⁺, Mn²⁺, Zn⁺, Fe³⁺, Co²⁺) at 50 °C for 10 min; CT means the contrast group. b Enzyme was incubated in 0–5 M NaCl at 50 °C for 10 min and determined the specific activity of the enzyme. The highest enzyme specific activity was taken as equivalent to 100% specific activity