Table 6 Combination of the experiment based on CCD for optimization of glucose concentration, peptone concentration, and pH level in the production of PHB by Bacillus cereus SH-02
Run no. | Factors | Response | |||
|---|---|---|---|---|---|
A:glucose concentration(g/L) | B:peptone concentration(g/L) | C:pH | PHB content(mg/L) | PHB concentration(%) | |
1 | 20 | 12.5 | 7 | 2880 | 26 |
2 | 30 | 5 | 8.5 | 300 | 5.5 |
3 | 20 | 12.5 | 7 | 2980 | 27 |
4 | 3.18207 | 12.5 | 7 | 82 | 1.4 |
5 | 20 | 12.5 | 7 | 2940 | 26.8 |
6 | 20 | 12.5 | 7 | 3051 | 28.7 |
7 | 30 | 5 | 5.5 | 1356 | 15.7 |
8 | 10 | 5 | 8.5 | 34 | 0.8 |
9 | 10 | 20 | 5.5 | 488 | 11.85 |
10 | 30 | 20 | 8.5 | 1954 | 20.1 |
11 | 20 | −0.113446 | 7 | 578 | 11.3 |
12 | 20 | 12.5 | 4.47731 | 890 | 13.3 |
13 | 20 | 25.1134 | 7 | 2152 | 19.8 |
14 | 10 | 5 | 5.5 | 340 | 5.4 |
15 | 30 | 20 | 5.5 | 1732 | 16.1 |
16 | 36.8179 | 12.5 | 7 | 1624 | 15 |
17 | 20 | 12.5 | 7 | 2860 | 28 |
18 | 20 | 12.5 | 7 | 2980 | 29.4 |
19 | 10 | 20 | 8.5 | 792 | 14.7 |
20 | 20 | 12.5 | 9.52269 | 802 | 12.7 |