Fig. 2

Significantly regulated proteins of A. salmonicida deletion variants exposed to Atlantic salmon serum. (A) Volcano plots showing the p-values of significance and log₂ fold change values comparing ΔA, ΔB or ΔAB against WT in the absence (left panel) and presence (right panel) of salmon serum (SS). Dotted line(s) traversing the y- and x-axis indicate the significance cutoff at p = 0.05 (-log₁₀ = 1.3) and ( ±) 1.5-fold change (log₂ = 0.58) in protein abundance. Significance was determined by a paired two-tailed t-test. (B) Heatmaps showing the fold change values (log₂) of significantly regulated proteins common for all deletion mutants. (C) Heatmap showing fold-change values (log₂) of significantly regulated proteins related to motility, chemotaxis, quorum sensing, protease activity and general stress response