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Fig. 3 | BMC Microbiology

Fig. 3

From: Cyclic-di-GMP stimulates keratinocyte innate immune responses and attenuates methicillin-resistant Staphylococcus aureus colonization in a murine skin wound infection model

Fig. 3

Stimulation of HaCaT cells with c-di-GMP induces gene expression of MHC class I and II. HaCaT cells were incubated for 12 h with c-di-GMP at 0, 1, 5, 25, or 125 μM and challenged with MRSA strain HRCH2019-1 for 3 h. Infected and uninfected control cells was harvested and total RNA was extracted with TRIzol. Relative gene expression of MHC class I and II was determined by quantitative real-time PCR following the 2.−ΔΔCt method with GAPDH expression for normalization. A Relative gene expression of MHC class I. B Relative gene expression of MHC class II. Significant differences compared with 0 μM c-di-GMP for both the MRSA-challenged and unchallenged cells were identified by two-way ANOVA with Dunnett’s multiple comparisons test (GraphPad Prism). ***, P < 0.001

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BMC Microbiology

ISSN: 1471-2180

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