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Fig. 1 | BMC Microbiology

Fig. 1

From: Cyclic-di-GMP stimulates keratinocyte innate immune responses and attenuates methicillin-resistant Staphylococcus aureus colonization in a murine skin wound infection model

Fig. 1

Stimulation of HaCaT cells with c-di-GMP inhibits MRSA colonization. A MRSA strain HRCH2019-1 was grown in TSB at 37 °C and 200 rpm in the presence of c-di-GMP (0, 1, 5, 25, or 125 μM) and bacterial growth was monitored by absorbance measurements (OD600) at 2-h intervals. B Cytotoxicity of c-di-GMP against HaCaT cells was determined with the CCK-8 assay. Viability of HaCaT cells after treatment for 24 h with c-di-GMP at 1, 5, 25 and 125 μM was determined relative to untreated control cells. C HaCaT cells were incubated for 12 h with c-di-GMP at 0, 1, 5, 25, or 125 μM and challenged with MRSA strain HRCH2019-1. Bacterial colonization of HaCaT cells was determined after 3 h incubation. Significant differences compared with 0 μM c-di-GMP were identified by one-way ANOVA with Dunnett’s multiple comparisons test (GraphPad Prism). ***, P < 0.001

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BMC Microbiology

ISSN: 1471-2180

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