Real-time PCR using atpE, conventional PCR targeting different regions of difference, and flow cytometry for confirmation of Mycobacterium bovis in buffaloes and cattle from the Delta area of Egypt

Table 7 Readings of different cell phenotypes from eight buffaloes and eight cattle on RPMI medium with different antigens for cellular stimulation

The antigens used for stimulation	Type of animal	Tuberculintest	Cell phenotypes
The antigens used for stimulation	Type of animal	Tuberculintest	CD4⁺	CD8⁺	WC1⁺δγ	CD2⁺
PPDB	Buffaloes	-ve	2233 ± 327^a	2016 ± 394^a	2000 ± 137^a	2666.7 ± 153^a
PPDB		+ve	3600 ± 255^b	2950 ± 28^b	2950 ± 375^b	2950 ± 259^b
MBP70		-ve	1833 ± 57^a	4250 ± 86^a	1300 ± 250^a	2366 ± 288^a
MBP70		+ve	2700 ± 404^b	4500 ± 808^b	2400 ± 115^b	3300 ± 115^b
ESAT6/CFP10		-ve	2900 ± 230^a	2760 ± 115^a	2500 ± 490^a	2250 ± 317^a
ESAT6/CFP10		+ve	3000 ± 404^b	3450 ± 144^b	2700 ± 173^b	2800 ± 115^b
PPDB	Cattle	-ve	6266.7 ± 296^a	5300 ± 472^a	6150 ± 683^a	4300 ± 827^a
PPDB		+ve	6666 ± 706^b	6350 ± 541^b	6550 ± 518^b	4950 ± 715b
MBP70		-ve	4750 ± 747^a	3900 ± 545^a	4850 ± 1007^a	4300 ± 626^a
MBP70		+ve	718 ± 5550^b	5916.7 ± 639^b	5966.7 ± 455^b	4650 ± 559^b
ESAT6/CFP10		-ve	5950 ± 836^a	4166.7 ± 492^a	5033.3 ± 709^a	4400 ± 89^a
ESAT6/CFP10		+ve	6416.7 ± 874^b	5866 ± 620^b	6150 ± 413^b	6300 ± 670^b

These data expressed as Mean ± SE of the percentage readings to 10,000 peripheral blood mononuclear cells (PBMC)
Each value represents the mean of 8 cases. Superscript letters a and b within the same column to the relevant species representing cases that are significantly different (p < 0.0001)

ISSN: 1471-2180