Real-time PCR using atpE, conventional PCR targeting different regions of difference, and flow cytometry for confirmation of Mycobacterium bovis in buffaloes and cattle from the Delta area of Egypt

Table 4 Results of tuberculin Test, postmortem examination, isolation, real-time PCR using atpE, and genomic RD-based PCR

Type of animal	Total no. of tested animals	Comparative intradermal tuberculin test	Sensitivity of tuberculin*	Specificity of tuberculin*	Postmortem examination		Culture on LJ with pyruvate**	Real-time PCR using atpE		RD based conventional PCR for confirmation of M. bovis
Type of animal	Total no. of tested animals	Comparative intradermal tuberculin test	Sensitivity of tuberculin*	Specificity of tuberculin*	Visible-lesion	Non visible-lesion	Culture on LJ with pyruvate**	Tissue	Culture	Tissue	Culture
Buffaloes	1500	18 (1.2%)	27.78%	100%	1/18 (5.6%)^a	17/18 (94.4%)^b	5/18 (27.8%)^a	18/18 (100%)^b	5/5 (100%)^b	14/18 (77.8%)^b	5/5 (100%)^b
Cattle	2200	36 (1.6%)	55.56%	100%	14/36 (38.9%)^a	36/22 (61.1%)^b	20/36 (55.6%)^a	36/36 (100%)^b	20/20 (100%)^b	30/36 (83.3%)^b	20/20 (100%)^b
Total	3700	54 (1.5%)	55.56%	100%	15/54 (27.8%)^a	39/54 (72.2%)^b	25/54 (46.3%) ^a	54/54 (100%)^b	25/25 (100%) ^b	44/54 (81.5%) ^b	25/25 (100%)^b

*The sensitivity and specificity of the comparative intradermal tuberculin test were calculated in relation to isolation
**All the cases showing gross lesions were positive for isolation, while all the non-visible cases were negative for isolation
Superscript letters a and b within the same row represent results that are significantly different (p < 0.0001)

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