Real-time PCR using atpE, conventional PCR targeting different regions of difference, and flow cytometry for confirmation of Mycobacterium bovis in buffaloes and cattle from the Delta area of Egypt

Table 3 Detailed descriptions of postmortem examination, M. bovis isolation, and results of single intradermal comparative cervical tuberculin test

Postmortem examination	Site of lesion	Number				Results of single intradermal comparative cervical tuberculin test
Postmortem examination	Site of lesion	Buffaloes	M. bovis isolation	Cattle	M. bovis isolation
1. Visible lesion (VL)	1.1localized					Skin thickness > 5 mm
	a. Head L.nods
	b. Lung and pulmonary L.nods	1/18 (5.6%)	1/18 (5.6%)	8/36 (22.2%)	8/36 (22.2%)
	c. Mesenteric L.nods
	1.2 Generalized^a			6/36 (16.7%)	6/14 (16.7%)
Total		1/18 (5.6%)		14/36 (38.9%)	14/36 (38.9%)
2. Non-visible lesion (NVL)	Congested L.nods	17/18 (94.4%)	4/18 (22.2%)	22/36 (61.1%)	6/36 (16.7%)	4 mm < Skin thickness < 5 mm
Total		18	5/18 (27.8%)	36	20/36 (55.6%)

^aGeneralized; Lesions found in lung and pulmonary L.nods with mesenteric L.nods of the same case

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