Type of PCR | Target gene | Primers and probe sequences (5′-3′) | Amplicon size (bp) | Annealing temperature (°C) | Melting temperature (°C) | Accession number | Reference |
---|---|---|---|---|---|---|---|
Real-time | atpE | F 5′-CGGGCCGGATCGGGA-3′ R 5′-CGAAGACGAACAGCCAT-3′ P FAM 5′-ACGTGATGAAGAACGGGT AA-3′ | 182 | 60 | 63.7 57.1 58.9 | CP023630.1 | [22] |
Conventionala | RD1 | 5′-AAGCGGTTGCCGCCGACCGACC-3′ 5′-CTGGCTATATTCCTGGGCCCGG-3′ 5′-GAGGCGATCTGGCGGTTTGGGG-3′ | 146 | 62 | 60.3 63.4 66 | CP009186.1 | [32] |
RD4 | 5′-ATGTGCGAGCTGAGCGATG-3′ 5′-TGTACTATGCTGACCCATGCG-3′ 5′-AAAGGAGCACCATCGTCCAC-3′ | 172 | 67.5 66.7 67.1 | CP009186.1 | |||
RD9 | 5′-CAAGTTGCCGTTTCGAGCC-3′ 5′-CAATGTTTGTTGCGCTGC-3′ 5′-GCTACCCTCGACCAAGTGTT-3′ | 235 | 66.6 63.9 66.5 | CP009186.1 | |||
RD12 | 5′-GGGAGCCCAGCATTTACCTC-3′ 5′-GTGTTGCGGGAATTACTCGG-3′ 5′-AGCAGGAGCGGTTGGATATTC-3′ | 369 |  | 67 65.7 66.9 | CP009186.1 |