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Fig. 3 | BMC Microbiology

Fig. 3

From: Real-time PCR using atpE, conventional PCR targeting different regions of difference, and flow cytometry for confirmation of Mycobacterium bovis in buffaloes and cattle from the Delta area of Egypt

Fig. 3

Amplification curve of real-time PCR assay using atpE primer/probe. The fluorescence increase wasn’t detectable until cycle 20 as enough amplified product was accumulated to yield a detectable fluorescence signal using control DNA with concentrations of (100,000, 1000, 100, 10, 1, 0.1, and 0.01 ng/μL). The crossing points and concentrations were automatically calculated by the real-time PCR software (LightCycler 480 Roche)

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BMC Microbiology

ISSN: 1471-2180

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