Fig. 2

Translation of the N-terminus of ermBL is critical for the induction of ermB expression by 16-membered ring macrolides. A The detailed RNA and amino acid sequence of ermBL and its own ribosome binding site (RBS1). B-E β-Galactosidase activity assay and disc diffusion assay of various ermBL mutants exposed to erythromycin, spiramycin and tylosin. Miller units of β-galactosidase activity are shown on the Y-axis. The number on the top of each bar represents the fold change in beta-gal activity between the antibiotic and DMSO group. The error bars correspond to the SEM of three independent experiments. All the β-Galactosidase activity of antibiotics group compared to DMSO group is significantly. P < 0.05; (unpaired two-tailed Student’s t test)