Table 3 Comparison of sequence, amplicon size, GC content and melting tempreture of specific PCR primers used for each pathogen in the MHRM analysis. List of primers used in this study and its properties
Pathogen | Gene Bank accession no | primer sequence/ Tm (°C) | Nucleotide positions | Amplicon size (bp) | GC content of amplicon (%) | Predicted Tma (°C) | Observed Tmb (°C) |
|---|---|---|---|---|---|---|---|
A. baumannii | CP000521 | F: GTGGCACATTAGGTCCCGA (56.4) R: CAAGGTAGTCTGCTTGAGTCG (58.4) | 3,143,607- 3,143,795 | 189 | 44 | 82.09 | 83.33 |
K. pneumoniae | CP077773 | F: GGCGAGGTTTACGTCTCAAC (55.9) R: GTACTTCTTGTTGGCCTCGC (56.2) | 5,129,431- 5,129,702 | 272 | 61 | 89.34 | 90.59 |
P. aeruginosa | CP050332 | F: ATCTTCTGGCTGTCTTCGGC (55.3) R: AATGTCCACCACGGTCTTCC (56.3) | 2,396,197- 2,396,388 | 192 | 70 | 92.77 | 94.57 |
S. aureus | CP053639 | F: GCTAAACCACTTTTGTTAGCACC (58.7) R: TGATAAAGAAAATGGCATGCACA (57.6) | 1,867,646- 1,867,798 | 153 | 31 | 76.44 | 78.1 |
E. coli | CP034658 | F: CATACCTGTTCACCGACGAC (55.4) R: CTGGCAGGAGAAACTGCATC (56.1) | 1,662,333- 1,662,506 | 174 | 53 | 84.95 | 86.74 |