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Table 1 Highest abundance proteins identified using AP-MS

From: Localization of EccA3 at the growing pole in Mycobacterium smegmatis

Uniprot

Annotation

MSMEG

Annotation

RV

homologue

Gene

Name

Protein Names

Gene Ontology

IFQ Intensity

A0QS98

MSMEG_1401

Rv0685

tuf

Elongation factor Tu (EF-Tu)

cytoplasm; GTPase activity; GTP binding; translation elongation factor activity

1,509,500,000

A0QQU5

MSMEG_0880

Rv0440

groL1

60 kDa chaperonin 1 (GroEL protein 1)

ATP binding; cytoplasm; protein refolding

464,740,000

A0R729

MSMEG_6759

 

glpK

Glycerol kinase

ATP binding; glycerol-3-phosphate metabolic process; glycerol catabolic process; glycerol kinase activity

257,030,000

A0R2Y1

MSMEG_5273

Rv1074c

fadA3

Acetyl-CoA acetyltransferase

transferase activity, transferring acyl groups other than amino-acyl groups

206,560,000

A0QSS4

MSMEG_1583

Rv3417c

groL2

60 kDa chaperonin 2 (GroEL protein 2)

ATP binding; cytoplasm; protein refolding

198,490,000

A0QWW2

MSMEG_3084

Rv1436

gap

Glyceraldehyde-3-phosphate dehydrogenase

cytoplasm; glucose metabolic process; glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; glycolytic process; NAD binding; NADP binding

189,420,000

A0QUX8

MSMEG_2374

Rv3001c

ilvC

Ketol-acid reductoisomerase

coenzyme binding; isoleucine biosynthetic process; ketol-acid reductoisomerase activity; valine biosynthetic process

183,580,000

A0QQC8

MSMEG_0709

Rv0350

dnaK

Chaperone protein DnaK

ATP binding; protein folding

141,160,000

A0QSS3

MSMEG_1582

Rv3418c

groS

10 kDa chaperonin (GroES protein)

ATP binding; cytoplasm; protein folding

132,270,000

A0R1H7

MSMEG_4757

Rv2524c

fas

Fatty acid synthase

enoyl-[acyl-carrier-protein] reductase (NADH) activity; fatty acid biosynthetic process; fatty acid synthase complex; nucleotide binding

101,460,000