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Fig. 3 | BMC Microbiology

Fig. 3

From: Localization of EccA3 at the growing pole in Mycobacterium smegmatis

Fig. 3

EccA3 localizes at the growing pole in Mycobacterium smegmatis. a Time-lapse imaging of eight independent clonal populations demonstrated that EccA3 localized at the old pole in 52 of the 73 cells imaged. b A single WTMS::pDMNI0615 cell with a fluorescent focus at the pole (i, white arrow) can be seen dividing into two cells (ii) with a red arrow at the septum. A second fluorescent foci representing EccA3-GFP can be seen at the old pole (iii, white arrow) before a third fluorescent foci (iv, white arrow) is detected following cell division (iv, red arrow at the septum). One of the fluorescent foci detected previously at the old pole (iii, white arrow) migrated to be polar adjacent (iv, white arrow)

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