Fig. 2
From: Localization of EccA3 at the growing pole in Mycobacterium smegmatis
Localization of EccA3 within a population of Mycobacterium smegmatis. Three independent clonal populations were used for imaging flow cytometry experiments. a Imaging flow cytometry was used to demonstrate that more than 60% of clonal populations of WTMS::pDMNI0615 and ΔESX-3MS::pDMNI0615 had a GFP focus at least 0.5 μm from the mid-cell when compared to no GFP (WTMS and ΔESX-3MS) and GFP positive controls (WTMS::pDMNI and ΔESX-3MS::pDMNI). Unipolar localisation was significant following a Kruskal-Wallis test with a p-value of 0.0036 for both WTMS and ΔESX-3MS (indicated with **). b The majority of GFP positive WTMS::pDMNI0615 and ΔESX-3MS::pDMNI0615 cells with a fluorescent focus at least 0.5 μm from the mid-cell had only one fluorescent focus. c The histogram shows distance of a fluorescent foci from the mid-cell of populations WTMS (red), WTMS::pDMNI (green), WTMS::pDMNI0615.1 (blue), WTMS::pDMNI0615.2 (blue-purple), WTMS::pDMNI0615.3 (purple). d The distance of fluorescent foci from the mid-cell of ΔESX-3MS (red), ΔESX-3MS::pDMNI (green), ΔESX-3MS::pDMNI0615.1 (blue), ΔESX-3MS::pDMNI0615.2 (blue-purple), ΔESX-3MS::pDMNI0615.3 (purple) populations. A population with GFP fluorescence located at least 0.5 μm from the mid-cell, represented by the red line called Delta, was used to score the number of fluorescent foci per cell