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Fig. 2 | BMC Microbiology

Fig. 2

From: Detection of Haemophilus influenzae by loop-mediated isothermal amplification coupled with nanoparticle-based lateral flow biosensor assay

Fig. 2

Optimization of amplification temperature for H. influenzae-LAMP assay. The LAMP method for H. influenzae detection was monitored with real-time turbidimeter. Eight temperatures and their corresponding curves were showed in the pictures. The turbidity > 0.1 was considered as the positive result (threshold value was 0.1). The H. influenzae DNA templates used in each reaction was 1 pg. Eight pictures (A-H) were acquired from a temperature range with 1℃ intervals (from 60℃ to 67℃). Signal 1, positive amplification of H. influenzae; signal 2, negative control of S. aureus

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BMC Microbiology

ISSN: 1471-2180

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