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Table 2 Frequency of IncX plasmid conjugative transfer under induced stress

From: Genomic analysis of qnr-harbouring IncX plasmids and their transferability within different hosts under induced stress

Mediaa

Tb

IncX1 (pHP2)

Standard deviation

IncX1 (pHP2 ΔqnrS)

Standard deviation

IncX2 (p194)

Standard deviation

IncX2 (p194 ΔqnrS)

Standard deviation

LB

37 °C

2.56 × 10–2

1.26 × 10–2

2.12 × 10–2

5.29 × 10–3

1.34 × 10–6

4.92 × 10–7

6.83 × 10–6

2.53 × 10–6

25 °C

1.45 × 10–4

5.95 × 10–5

3.84 × 10–4

9.71 × 10–5

2.77 × 10–4

2.25 × 10–4

1.52 × 10–3

2.41 × 10–4

LB—CIP 0.001

37 °C

2.63 × 10–2

1.22 × 10–2

1.39 × 10–2

2.85 × 10–3

3.47 × 10–5

3.24 × 10–6

7.09 × 10–6

2.63 × 10–6

25 °C

4.85 × 10–4

4.24 × 10–5

2.27 × 10–4

6.19 × 10–5

8.77 × 10–4

2.05 × 10–4

1.42 × 10–2

4.11 × 10–3

LB—CIP 0.05

37 °C

3.34 × 10–3

1.19 × 10–3

2.76 × 10–1

5.06 × 10–2

1.32 × 10–6

1.56 × 10–7

1.34 × 10–6

7.78 × 10–7

25 °C

4.25 × 10–4

5.34 × 10–5

3.43 × 10–2

4.96 × 10–3

4.01 × 10–4

1.24 × 10–4

1.67 × 10–2

2.40 × 10–3

LB—CIP 0.5

37 °C

2.11 × 10–2

4.70 × 10–3

9.21 × 10–2

1.82 × 10–2

2.52 × 10–6

1.94 × 10–6

4.10 × 10–6

2.18 × 10–6

25 °C

1.17 × 10–4

3.13 × 10–5

2.38 × 10–1

4.51 × 10–2

9.23 × 10–5

2.30 × 10–5

4.55 × 10–5

5.65 × 10–5

LB—CIP 2

37 °C

3.12 × 10–2

8.22 × 10–3

1.50 × 10–0

1.61 × 10–1

4.07 × 10–8

7.04 × 10–8

4.45 × 10–5

3.08 × 10–5

25 °C

2.71 × 10–4

3.71 × 10–5

4.16 × 10–1

1.92 × 10–1

1.37 × 10–5

2.37 × 10–6

5.14 × 10–4

1.24 × 10–4

  1. Frequency of transfer was calculated as the number of transconjugants per the number of donors (T/D)
  2. aLB stands for Luria–Bertani agar. CIP represents the concentration (µg/mL) of supplemented ciprofloxacin into the media
  3. bT represents the incubation temperature during conjugation