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Table 4 Results from 10 PCR-positive clinical specimens with suspected endocarditis

From: Comparison of an automated DNA extraction and 16S rDNA real time PCR/sequencing diagnostic method using optimized reagents with culture during a 15-month study using specimens from sterile body sites

Material Clinical information PCR Culture Antibioticsb Microscopy Cp and copy number
Valve replacement with vegetations acute “prosthetic valve endocarditis” after dentist Cutibacterium acnes negative (blood culture not available) Augmentin, rimactan, zyvoxid negative Cp 30.44
3 copies 16S rDNA
Aortic valve severe aortic regurgitation with thrombus situation after embolization duration of illness: 4 months Cardiobacterium hominis negative (blood culture not available) none negative Cp 24.79
3 copies 16SrDNA
Aortic valve endocarditis with S.mutans Streptococcus mutans negative (blood cultures with S.mutans 18 days ago) Penicillin G, gentamycin negative Cp 22.94
5 copies 16S rDNA
Aortic valve both valves involved mitral valve: PCR negative Staphylococus coagulase negative negative (blood culture not available) Floxapen (since 2 days) negative Cp 26.70
~ 6 copies 16S rDNA
Aortic valve endocarditis aortic and mitral valve Staphylococcus epidermidis S. epidermidis (only in enrichment culture)
(blood cultures with S.epidermidis 2 and 3 days ago)
Vanco, tazobac not done Cp 24.57
6 copies 16S rDNA
Mitral valve endocarditis mitral valve with E.faecalis Enterococcus faecalis E. faecalis (only in anaerobic culture)
(blood culture with E.faecalis analyzed previously by another laboratory)
Clamoxyl/vancocin negative Cp 25.47
4 copies 16S rDNA
Mitral valvea endocarditis mitral valve Streptococcus mitis group negative (blood culture not available) no information gram pos. Cocci ++ Cp 22.36
4 copies 16S rDNA
Tricuspid valvea endocarditis mitral valve Streptococcus mitis group negative (blood culture not available) no information gram pos. Cocci ++ Cp 23.13
4 copies 16S rDNA
Aortic valve bicuspid aortic valve endocarditis Abiotropha defectiva negative (blood culture not available) no information negative Cp 27.63
4 copies 16S rDNA
Mitral valve severe insufficiency of mitral valve Streptococcus mutans negative (blood culture not available) no information gram pos. Cocci ++ Cp 27.35
5 copies 16S rDNA
  1. Clinical information according to the client. Culture includes aerobic incubation on plates, anaerobic incubation on plates and enrichment culture with liquid medium. Previously taken blood cultures are listed additionally. The date of positive blood culture refers to the date of registration of clinical specimens in our laboratory
  2. aSame patient
  3. bPrior to sending to the laboratory, according to the client