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Fig. 2 | BMC Microbiology

Fig. 2

From: Comparison of an automated DNA extraction and 16S rDNA real time PCR/sequencing diagnostic method using optimized reagents with culture during a 15-month study using specimens from sterile body sites

Fig. 2

Example of a mixed chromatogram. RiSeq results show the presence of Citrobacter koseri and coagulase-negative Staphylococcus (Case 2,111,805,855). Culture confirmed only Citrobacter koseri. The material was a biopsy of the malleolus, and the patient was treated with augmentin. Because Sanger sequencing mainly detects concentration differences of approximately less than 1:10 [13], this must be a relevant mixed infection due to a Cp of 29.9 of the amplification. In addition, the same patient had a second clinical specimen with the same mixed sequence. Chromatogram shows stretches of sequence diversity and sequence identity of the two strains. Example is after the 15 months study period

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BMC Microbiology

ISSN: 1471-2180

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