Table 1 Selection of relevant parameters in appraisal for screening of differentially expressed protein

Enzyme

Trypsin

Max Missed Cleavages

2

Fixed modifications

Carbamidomethyl (C), iTRAQ 8plex (N-term), iTRAQ 8plex (K)

Variable modifications

Oxidation (M), iTRAQ 8plex (Y)

Peptide Mass Tolerance

 ± 20 mg/Kg

Fragment Mass Tolerance

0.1 Da

Database

uniprot_Nicotiana tabacum

Peptide FDR

 ≤ 0.01

T-test

p ≤ 0.05

Fold change

 ≥ 1.2 or ≤ 0.83