Modulation of microRNAs and claudin-7 in Caco-2 cell line treated with Blastocystis sp., subtype 3 soluble total antigen

Table 1 Stem-loop RT primers designed for each studied miRNA and its forward real-time PCR primer

Gene name	Primers		Tm (°C)
Hsa-16-5p	Real-time	ACACTCCAGATGGGTAGCAGCACGTAA	60
Hsa-16-5p	RT	CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCGCCAATA	60
Hsa -29a-3p	Real-time	ACTCTCGAGCACTGTAGCACCATCTGAA	60
Hsa -29a-3p	RT	CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTAACCGAT	60
Hsa −21-5p	Real-time	ACACTCCAACAGGGTAGCTTATCAGACT	60
Hsa −21-5p	RT	CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTCAACATC	60
Hsa −223-3p	Real-time	AGTCTCCAGCAGGGTGTCAGTTTGTCAA	60
Hsa −223-3p	RT	CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTGGGGTAT	60
Hsa −874-3p	Real-time	ACACTCCTGCTGCGCTGCCCTGGCCCGA	60
Hsa −874-3p	RT	CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTCGGTCCC	60
U6	Real-time	ACACTCCATCTGGGTCGTGAAGCGTTC	60
U6	RT	CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAAAAATATG	60
Universal reverse	Real-time	TGGTGTCGTGGAGTCGGCAATTCAGTTG	60

ISSN: 1471-2180