Cryopreservation of vegetative cells and zygotes of the multicellular volvocine green alga Gonium pectorale

BMC Microbiology

Table 4 Comparison of zygote germination rates of Gonium pectorale strains NIES-4501 × NIES-4502 after cryopreservation among six types of cryoprotectant conditions

Cryoprotectant	Zygote germination rate (rates in three PCR tubes) [%]	Control zygote germination rate^a [%]
5% DMSO	2.9 ± 2.9 (2.8, 5.8, 0)^b	77
10% DMSO	26 ± 7.9 (26, 19, 34)^b	72
5% Methanol	61 ± 8.7 (70, 52, 60)^b	67
10% Methanol	59 ± 2.0 (61, 57, 60)^b	61
5% DMF	0.42 ± 0.73 (1.3, 0, 0)^b	76
10% DMF	5.9 ± 5.2 (12, 3.1, 2.7)^b	69
No cryoprotectant	0.0 ± 0.0 (0, 0, 0)	78

^a Without cryopreservation
^b Significant difference (p < 0.01) was detected between three types of cryoprotectants based on unweighted-mean ANOVA analyzed by js-STAR XR release 1.6.6j < http://www.kisnet.or.jp/nappa/software/star/index.htm >

ISSN: 1471-2180