Cryopreservation of vegetative cells and zygotes of the multicellular volvocine green alga Gonium pectorale

BMC Microbiology

Table 3 Comparison of results of strains of five other species of Gonium (Additional file 1: Table S1) after possible optimal cryogenic treatment (1.0 mL 6% DMF in 2 mL cryotube; Table 1) in liquid nitrogen

Species	Strain designation	Survivability based on MPN method [%]	Number of viable cultures by 1st [2nd] inoculation with 3 [3] 10 mL cultures
G. maiaprilis	NIES-2455	0.00043 ± 0.00075	1[0]
G. maiaprilis	NIES-2456	0	0[0]
G. multicoccum	NIES-737	0	0[0]
G. multicoccum	NIES-885	0	1[0]
G. octonarium	NIES-851	0.054 ± 0.042	3[1]
G. octonarium	NIES-852	0.0035 ± 0.049	3[0]
G. quadratum	NIES-652	0	0[0]
G. quadratum	NIES-653	0	1[0]
G. viridistellatum	NIES-288	0.13 ± 0.23	3[1]
	NIES-290	0.0022 ± 0.0019	3[1]
	NIES-654	0	0[0]
	NIES-655	0	0[0]

ISSN: 1471-2180