Using unique ORFan genes as strain-specific identifiers for Escherichia coli

BMC Microbiology

Table 3 List of strain-specific qPCR primers

Strain^a	Gene locus tag	Primers		Ann. T° (°C)	Product (bp)
Strain^a	Gene locus tag	Forward (5′ → 3′)	Reverse (5′ → 3′)	Ann. T° (°C)	Product (bp)
ChH1	ECMDIDHM_04458	CGCTACCAGGGACAGTACCT	TGATAGAGATTCAGTCCCCCCG	60	132
ChH2	ANLLMAEJ_01457	ACGAATGTGACCGAGCAGAG	CAGCGTACACCGAGTAAAACC	60	159
ChH3	PDAKODAI_04433	CGGAATTACCGTCGTAGAGCAG	CAGCACGATCACCAGAATAGAAGTG	60	115
C1	KGJMKMMK_04916	TGACAGCGAAAACCCAGCTC	CAGTTTGCCCCTGGATTTCC	60	151
C2	KGPFHGEA_00668	TGCGTTTGCAATTTACGGCG	GCGGCTCTATCCTTTGAGTCG	61	198
C3	AMKFMELI_04046	CAAACTCGACAAGAGCAACGC	AGAAGCAAAGAAACCGCCCC	61	198
C4	LCFLNNEA_01211	GCGAATTGCCAAAGAAAGCCAG	TGCGGATATGCAGCAAATCTCC	60	108
C5	GKDLAHGH_04328	GGCAGGGCCAAGCTTTAGTAC	GAGCTGCAAAACATGCCCATAC	61	173
C6	LDHELLGP_02401	GGCTGCGGAAGAGTGTAAGTT	GCGGCTCATATTTTTCATCAGCGTC	61	182
P1	NIHDEHIB_01629	CAACGAGTTAGCGGAACTCCA	CTTCTTGGCAATCAGCACAGC	60	161
P2	EKBHOPAC_04494	GAGTGGAGCCATGACTTCTGC	CCAAACGCCTAATATTTCTGCGACA	61	157
P3	DGMDDDKP_04627	CATGTCTTCTAATGGCGGTCGT	GTGCAACGGTCTAACATTCGCT	60	123
P4	LLPKFPJB_0002	CGCAGACTCTATTGCGTCTGG	GGACTTGCGATGTAGAATCCAATC	60	132
P5	HEMKEPCD_04830	AGCGGGCCGATGACAAATAC	TACAGGAAGCCGATAACCCCAC	61	184
M1	PJHDGJGH_02587	CTTGCGATTGAATCTGGCAGTGT	CACACCAGCATCTATTAAGCCCTG	60	168
M2	HDADFJHI_04570	CGACTGGCGCAATAACCAC	AGCACACCCGTCTTCATCATC	60	151
M3	EBAAKEFM_00440	CGGCACAGGCGGAAAAAAC	CGTCACCTCGTCTCCAAACATAAAG	61	250
uidA^b	–	GCGAGGTACGGTAGGAGTTG	GAAGGGCGAACAGTTCCTGA	60	101

ISSN: 1471-2180