Table 1 Genes, putative function of products, primers, annealing temperatures and predicted size of amplification products
Gene | Product | Primer sequence (5′-3′) | Product size (bp) | Annealing temperature (°C) | Reference |
|---|---|---|---|---|---|
pap | P-fimbriae | GACGGCTGTACTGCAGGGTGTGGCG/ ATATCCTTTCTGCAGGGATGCAATA | 328 | 65 | [15] |
hlyA | Hemolysin A | TTGAGTCACACCTGGGAGAC/ CCGTGTAATTACCCGCTTCG | 162 | 57 | This study |
cnf1 | Cytotoxic necrotizing factor 1 | TGCGGGTGTAAATTCAGTGC/ TCTCGTTGAGCCTCACTGTT | 186 | 56 | This study |
hlyE | Hemolysin E | GAAACCGCAGATGGAGCATT/ CGCAACACCACACCATTCAT | 219 | 56 | This study |
16S rRNA | Ribosomal RNA | GAATGCCACGGTGAATACGTT/ ACCCACTCCCATGGTGTGA | 64 | 57 | [16] |