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Table 2 Box-Behnken factorial experimental design for melanin pigment production by Hortaea werneckii AS1

From: Optimization of melanin pigment production from the halotolerant black yeast Hortaea werneckii AS1 isolated from solar salter in Alexandria

Runs

Experimental parameters

Response (g/L)

 

CaCl2 (g/L)

Trace element (mL/L)

Culture volume (mL/500mL)

1

0[0.75]

0[0.5]

0[150]

0.58

2

-1[0.375]

1[0.75]

0[150]

0.59

3

0[0.75]

-1[0.25]

-1[75]

0.44

4

1[1.125]

0[0.5]

-1[75]

0.51

5

0[0.75]

1[0.75]

-1[75]

0.41

6

1[1.125]

-1[0.25]

0[150]

1.01

7

-1[0.375]

-1[0.25]

0[150]

0.44

8

0[0.75]

1[0.75]

1[225]

0.8

9

1[1.125]

0[0.5]

1[225]

0.9

10

0[0.75]

0[0.5]

0[150]

0.69

11

0[0.75]

-1[0.25]

1[225]

0.77

12

-1[0.375]

0[0.5]

-1[75]

0.28

13

-1[0.375]

0[0.5]

1[225]

1

14

1[1.125]

1[0.75]

0[150]

0.98

15

0[0.75]

0[0.5]

0[150]

0.65