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Table 2 Box-Behnken factorial experimental design for melanin pigment production by Hortaea werneckii AS1

From: Optimization of melanin pigment production from the halotolerant black yeast Hortaea werneckii AS1 isolated from solar salter in Alexandria

Runs Experimental parameters Response (g/L)
  CaCl2 (g/L) Trace element (mL/L) Culture volume (mL/500mL)
1 0[0.75] 0[0.5] 0[150] 0.58
2 -1[0.375] 1[0.75] 0[150] 0.59
3 0[0.75] -1[0.25] -1[75] 0.44
4 1[1.125] 0[0.5] -1[75] 0.51
5 0[0.75] 1[0.75] -1[75] 0.41
6 1[1.125] -1[0.25] 0[150] 1.01
7 -1[0.375] -1[0.25] 0[150] 0.44
8 0[0.75] 1[0.75] 1[225] 0.8
9 1[1.125] 0[0.5] 1[225] 0.9
10 0[0.75] 0[0.5] 0[150] 0.69
11 0[0.75] -1[0.25] 1[225] 0.77
12 -1[0.375] 0[0.5] -1[75] 0.28
13 -1[0.375] 0[0.5] 1[225] 1
14 1[1.125] 1[0.75] 0[150] 0.98
15 0[0.75] 0[0.5] 0[150] 0.65