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Table 1 The applied Plackett–Burman experimental design and the statistical analysis for elven cultural variables for the production of melanin pigment by H. werneckii AS1

From: Optimization of melanin pigment production from the halotolerant black yeast Hortaea werneckii AS1 isolated from solar salter in Alexandria

Trial Factor symbol Response
  Glucose (g/L) KNO3 (g/L) K2HPO4 (g/L) CaCl2 (g/L) MgSO4 (g/L) Tyrosine (g\L) Trace element (mL/L) pH Culture age (day) Inoculum size (mL/L) Volume (mL/500mL) Melanin (g/L)
1 −1[10] −1[7.5] −1[1.25] +1[0.75] +1[1.5] +1[1.5] −1[0.5] +1[6.5] +1[10] −1[0.5] +1[150] 0.538
2 −1[10] −1[7.5] +1[3.75] +1[0.75] +1[1.5] −1[0.5] +1[1.5] +1[6.5] −1[4] +1[1.5] −1[50] 0.217
3 −1[10] +1[22.5] −1[1.25] −1[0.25] −1[0.5] +1[1.5] +1[1.5] +1[6.5] −1[4] +1[1.5] +1[150] 0.327
4 +1[30] +1[22.5] −1[1.25] +1[0.75] −1[0.5] −1[0.5] −1[0.5] +1[6.5] +1[10] +1[1.5] −1[50] 0.531
5 −1[10] +1[22.5] +1[3.75] −1[0.25] +1[1.5] −1[0.5] −1[0.5] −1[4.5] +1[10] +1[1.5] +1[150] 0.340
6 +1[30] −1[7.5] +1[3.75] +1[0.75] −1[0.5] +1[1.5] −1[0.5] −1[4.5] −1[4] +1[1.5] +1[150] 0.876
7 +1[30] −1[7.5] +1[3.75] −1[0.25] −1[0.5] −1[0.5] +1[1.5] +1[6.5] +1[10] −1[0.5] +1[150] 0.219
8 +1[30] −1[7.5] −1[1.25] −1[0.25] +1[1.5] +1[1.5] +1[1.5] −1[4.5] +1[10] +1[1.5] −1[50] 0.029
9 +1[30] +1[22.5] +1[3.75] −1[0.25] +1[1.5] +1[1.5] −1[0.5] +1[6.5] −1[4] −1[0.5] −1[50] 0.046
10 −1[10] −1[7.5] −1[1.25] −1[0.25] −1[0.5] −1[0.5] −1[0.5] −1[4.5] −1[4] −1[0.5] −1[50] 0.091
11 −1[10] +1[22.5] +1[3.75] +1[0.75] −1[0.5] +1[1.5] +1[1.5] −1[4.5] +1[10] −1[0.5] −1[50] 0.153
12 +1[30] +1[22.5] −1[1.25] +1[0.75] +1[1.5] −1[0.5] +1[1.5] −1[4.5] −1[4] −1[0.5] +1[150] 0.598
13 0[20] 0[15] 0[2.5] 0[0.5] 0[1] 0[1] 0[1] 0[5.5] 0[7] 0[1] 0[100] 0.431
Main effect 0.11 0.004 -0.044 0.31 -0.07 -0.005 -0.15 -0.035 -0.06 0.11 0.31  
t-value 0.69 0.02 0.28 2.53 0.46 0.02 1.0 0.22 0.37 0.73 2.47  
  1. t-value significant at the 1% level = 3.70
  2. t-value significant at the 5% level = 2.446
  3. t-value significant at the 10% level = 1.94
  4. t-value significant at the 20% level =1.372
  5. Standard t-values are obtained from Statistical Methods [37]