Table 1 The applied Plackett–Burman experimental design and the statistical analysis for elven cultural variables for the production of melanin pigment by H. werneckii AS1
Trial | Factor symbol | Response | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Â | Glucose (g/L) | KNO3 (g/L) | K2HPO4 (g/L) | CaCl2 (g/L) | MgSO4 (g/L) | Tyrosine (g\L) | Trace element (mL/L) | pH | Culture age (day) | Inoculum size (mL/L) | Volume (mL/500mL) | Melanin (g/L) |
1 | −1[10] | −1[7.5] | −1[1.25] | +1[0.75] | +1[1.5] | +1[1.5] | −1[0.5] | +1[6.5] | +1[10] | −1[0.5] | +1[150] | 0.538 |
2 | −1[10] | −1[7.5] | +1[3.75] | +1[0.75] | +1[1.5] | −1[0.5] | +1[1.5] | +1[6.5] | −1[4] | +1[1.5] | −1[50] | 0.217 |
3 | −1[10] | +1[22.5] | −1[1.25] | −1[0.25] | −1[0.5] | +1[1.5] | +1[1.5] | +1[6.5] | −1[4] | +1[1.5] | +1[150] | 0.327 |
4 | +1[30] | +1[22.5] | −1[1.25] | +1[0.75] | −1[0.5] | −1[0.5] | −1[0.5] | +1[6.5] | +1[10] | +1[1.5] | −1[50] | 0.531 |
5 | −1[10] | +1[22.5] | +1[3.75] | −1[0.25] | +1[1.5] | −1[0.5] | −1[0.5] | −1[4.5] | +1[10] | +1[1.5] | +1[150] | 0.340 |
6 | +1[30] | −1[7.5] | +1[3.75] | +1[0.75] | −1[0.5] | +1[1.5] | −1[0.5] | −1[4.5] | −1[4] | +1[1.5] | +1[150] | 0.876 |
7 | +1[30] | −1[7.5] | +1[3.75] | −1[0.25] | −1[0.5] | −1[0.5] | +1[1.5] | +1[6.5] | +1[10] | −1[0.5] | +1[150] | 0.219 |
8 | +1[30] | −1[7.5] | −1[1.25] | −1[0.25] | +1[1.5] | +1[1.5] | +1[1.5] | −1[4.5] | +1[10] | +1[1.5] | −1[50] | 0.029 |
9 | +1[30] | +1[22.5] | +1[3.75] | −1[0.25] | +1[1.5] | +1[1.5] | −1[0.5] | +1[6.5] | −1[4] | −1[0.5] | −1[50] | 0.046 |
10 | −1[10] | −1[7.5] | −1[1.25] | −1[0.25] | −1[0.5] | −1[0.5] | −1[0.5] | −1[4.5] | −1[4] | −1[0.5] | −1[50] | 0.091 |
11 | −1[10] | +1[22.5] | +1[3.75] | +1[0.75] | −1[0.5] | +1[1.5] | +1[1.5] | −1[4.5] | +1[10] | −1[0.5] | −1[50] | 0.153 |
12 | +1[30] | +1[22.5] | −1[1.25] | +1[0.75] | +1[1.5] | −1[0.5] | +1[1.5] | −1[4.5] | −1[4] | −1[0.5] | +1[150] | 0.598 |
13 | 0[20] | 0[15] | 0[2.5] | 0[0.5] | 0[1] | 0[1] | 0[1] | 0[5.5] | 0[7] | 0[1] | 0[100] | 0.431 |
Main effect | 0.11 | 0.004 | -0.044 | 0.31 | -0.07 | -0.005 | -0.15 | -0.035 | -0.06 | 0.11 | 0.31 | Â |
t-value | 0.69 | 0.02 | 0.28 | 2.53 | 0.46 | 0.02 | 1.0 | 0.22 | 0.37 | 0.73 | 2.47 | Â |