Fig. 5

Enhanced Gag-Pol autocleavage reduced Gag-Pol trans incorporation into Gag particles. a Schematic representations of HIV-1 Gag and Gag-Pol expression constructs. Designated constructs are identical to those shown in Fig. 4a, but expressed in a gag/pol frameshift mutation backbone (GPfs). b-c 293T cells were transfect with WT or W402A or co-transfected with the designated construct plus a Pr55gag (Gag) expression vector at a plasmid DNA ratio of 1:10. SK denotes the plasmid pBluescript. At 48–72 h post-transfection, cells and supernatants were collected, prepared, and subjected to Western immunoblotting. Relative levels of virus-associated RT are indicated at bottom. d Relative levels of virus-associated RT were determined as described in the Fig. 4 caption. All data are from three independent experiments. Error bars indicate standard deviation *p < 0.05