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Fig. 4 | BMC Microbiology

Fig. 4

From: Effects of reduced gag cleavage efficiency on HIV-1 Gag-Pol package

Fig. 4

Virus yields and Gag-Pol packaging reduced by enhanced Gag-Pol autocleavage. a Schematic representations of HIV-1 Gag and Gag-Pol expression constructs. “X” denotes a PR-inactivated mutation. p6* adjacent to the active PR domain was replaced with a leucine zipper (LZ) as denoted by the striped (Wz) box. Remaining C-terminal p6* residues are in boldface. Arrows denote PR cleavage sites. Altered or additional residues are in italics. b 293T cells were transfected with designated constructs. Two days post-transfection, cells and supernatants were collected, prepared, and subjected to Western immunoblotting. c Relative levels of virus-associated RT. Levels of HIV-1 p24gag-associated Gag proteins Pr55, p41, and p24 and RT-associated Pol proteins Gag-Pol, Pol and p66/51RT were quantified by scanning immunoblot band densities. Ratios of total virus-associated RT versus Gag protein levels were calculated for each sample and normalized to the wt in parallel experiments. All data were obtained from three independent experiments. Error bars indicate standard deviation *p < 0.05

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