Table 1 Treatment groups. Dosages and routes for drug administration of sibutramine and FK506 were based upon previous studies (Additional Fig. 1), while bupropion and naltrexone dosages were adapted from the literature [24, 25]. Each animal received drug and/or vehicle in a total volume of 4 ml/kg po (treatment 1) and 2 ml/kg ip (treatment 2)

A

vehicle (castor oil/dehydrated alcohol po, 4 ml/kg po)

vehicle (saline, 2 ml/kg ip) (Fig. S1)

Fig S1

12

B

FK506 (0.6 mg/kg po)

vehicle (saline, 2 ml/kg ip) (Fig. S1)

Fig S1

10

C

vehicle (castor oil/dehydrated alcohol, 4 ml/kg po)

bupropion (20 mg/kg ip)

[24]

10

D

vehicle (castor oil/dehydrated alcohol, 4 ml/kg po)

naltrexone (1 mg/kg ip)

[26]

10

E

FK506 (0.6 mg/kg po)

bupropion (20 mg/kg ip)

Fig S1 [24]

10

F

FK506 (0.6 mg/kg po)

naltrexone (1 mg/kg ip)

Fig S1 [26]

10

G

vehicle (castor oil/dehydrated alcohol, 4 ml/kg po)

bupropion (20 mg/kg ip) plus

naltrexone (1 mg/kg ip)

[26]

10

H

sibutramine (5 mg/kg po)

vehicle (saline, 2 ml/kg ip)

Fig S1

10