Table 4 Bacterial plasmids
From: LysX2 is a Mycobacterium tuberculosis membrane protein with an extracytoplasmic MprF-like domain
Bacterial plasmidsa | Relevant characteristics | Source or reference |
|---|---|---|
pCR-Blunt II-TOPO | ccdB lethal gene ORF | Invitrogen Life Technology |
pSM316 | Integrative vector | [55] |
pUCCMPHOA | Integrative vector for in frame fusions to phoA gene of E. coli | [52] |
pJF751 | Integrative vector for in frame fusions to lacZ gene of E. coli | [24] |
pFRA30 (A) | Mt_lysX2 (A111) cloned in pUCCMPHOA | This work |
pFRA29 (A) | Mt_lysX2 (A111) cloned in pJF751 | This work |
pFRA31 (B) | Mt_lysX2 (H224) cloned in pUCCMPHOA | This work |
pFRA32 (B) | Mt_lysX2 (H224) cloned in pJF751 | This work |
pFRA35 (C) | Mt_lysX2 (L430) cloned in pUCCMPHOA | This work |
pFRA36 (C) | Mt_lysX2 (L430) cloned in pJF751 | This work |
pFRA144(D) | Mt_lysX (P259) cloned in pUCCMPHOA | This work |
pFRA162 (D) | Mt_lysX (P259) cloned in pJF751 | This work |
pFRA159 (E) | Mt_lysX (L488) cloned in pUCCMPHOA | This work |
pFRA156 (E) | Mt_lysX (L488) cloned in pJF751 | This work |
pFRA160(F) | Mt_lysX (S592) cloned in pUCCMPHOA | This work |
pFRA157 (F) | Mt_lysX (S592) cloned in pJF751 | This work |
pFRA161 (G) | Mt_lysX (Q699) cloned in pUCCMPHOA | This work |
pFRA158 (G) | Mt_lysX (Q699) cloned in pJF751 | This work |
pFRA176 (H) | Sa_mprF(S521) cloned in pUCCMPHOA | This work |
pFRA177 (H) | Sa_mprF(S521) cloned in pJF751 | This work |
pFRA178 (I) | Sa_mprF(F615) cloned in pUCCMPHOA | This work |
pFRA179 (I) | Sa_mprF(F615) cloned in pJF751 | This work |
pROL-HYG | replicative plasmid | [37] |
pROL-HYG::Mt_lysX2 | Mt_lysX2 + 543 bp upstream cloned inpROL-HYG | This work |